龙峰等：Development of chemiluminescent lab-on-fiber immunosensor for rapid point-of-care testing of anti-SARS-CoV-2 antibodies and evaluation of longitudinal immune response kinetics following three-dose inactivation virus vaccination
发布时间：2022-10-20
摘要：
新冠疫情严重影响人们健康和经济发展，接种疫苗可减少感染并抑制新冠病毒的传播。特异性抗体的定量检测对于评估疫苗接种后的免疫获得和长程保护效果，以及根据群体免疫水平改进疫苗接种部署极为重要，因此，亟需发展可现场即时检测的SARS-CoV-2特异性抗体的Point-of-care testing (POCT)技术。龙峰教授研究团队的徐文娟博士等研发了一款新型化学发光lab-on-fiber免疫传感器（chemiluminescent lab-on-fiber immunosensor，c-LOFI）。该仪器采用功能化光纤生物探针作为生物识别和分离器件，基于免疫夹心法，实现了SARS-CoV-2 IgG和IgM的高灵敏检测。同时，该技术也被成功地应用于全血样本中的SARS-CoV-2抗体的直接检测，仅需将指尖血进行简单稀释即可实现抗体的快速检测。更为重要的是，基于SARS-CoV-2 IgG检测结果，开展了三剂灭活病毒疫苗接种后的长期（>12个月）免疫反应动力学研究，其结果对解析新冠肺炎的免疫机制以及确定可能受益于疫苗接种和强化疫苗接种人群具有重要意义。该技术利用功能化光纤探针替代传统的免疫磁珠，实现靶标物的高效捕获、浓缩和分离，极大地提高了化学发光免疫分析效率和灵敏度，有望成为一款灵敏、低成本、快速、高频检测的POCT工具，为疫情防控和精准医疗提供新的技术手段。
Abstract:
Developing reliable, rapid, and quantitative point-of-care testing (POCT) technology of SARS-CoV-2-specific antibodies and understanding longitudinal vaccination response kinetics are highly required to restrain the ongoing coronavirus disease 2019 (COVID-19) pandemic. We demonstrate a novel portable, sensitive, and rapid chemiluminescent lab-on-fiber detection platform for detection of anti-SARS-CoV-2 antibodies: the chemiluminescent lab-on-fiber immunosensor (c-LOFI). Using SARS-CoV-2 Spike S1 RBD protein functionalized fiber bio-probe, the c-LOFI can detect anti-SARS-CoV-2 immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies with high sensitivity based on their respective horseradish peroxidase-labeled secondary antibodies. The limits of detection of anti-SARS-CoV-2 IgG and IgM antibodies were 0.6 and 0.3 ng/ml, respectively. The c-LOFI was successfully applied for direct detection of anti-SARS-CoV-2 antibodies in whole blood samples with simple dilution, which can serve as a finger prick test to rapidly detect antibodies. Furthermore, the longitudinal immune response (>12 months) kinetics following three-dose inactivated virus vaccines was evaluated based on anti-SARS-CoV-2 IgG detection results, which can provide important significance for understanding the immune mechanism against COVID-19 and identify individuals who may benefit from the vaccination and booster vaccination. The c-LOFI has great potential to become a sensitive, low-cost, rapid, high-frequency POCT tool for the detection of both SARS-CoV-2-specific antibodies and other biomarkers.
文章来源：Journal of Medical Virology（最新影响因子为20.693）